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KMID : 0853020110140020093
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Journal of Korean Burn Society
2011 Volume.14 No. 2 p.93 ~ p.96
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The Rapid Establishment of Human Clonal Adipose Derived Stem Cell (hADSC) Lines with Aspirated Adipose Tissue
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Park Soo-Jeong
Lee Su-Man
Lee Kyo-Won
Lim Dae-Seog
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Abstract
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Purpose: This study aims to establish a new strategy that provides for the rapid establishment of human clonal adipose derived stem cell (hADSC) lines with aspirated adipose tissue and to characterize newly generated hMSC lines for their cell phenotype, differentiation potential, lineage-specific gene expression.
Methods: Human adipose tissue-derived stem cells (hADSCs) were isolated from subcutaneous adipose tissue based on standard protocols. After incubation for 2 h, only the cell culture supernatant was transferred to a new dish. This process was repeated several times with 30 h incubations.
Results: We confirmed the difference in growth rate, however, differences were not seen in the differentiation capabilities and stemness of the each cell lines.
Conclusion: It is necessary to establish cell lines via single cell level for application to disease specific tissue engineering.
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KEYWORD
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Human adipose-derived stem cells, Human mesenchymal stem cells, Lineage-specific gene expression
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